Hot take: Ace Ventura: Pet Detective isn't necessarily transphobic.
The movie invites us to laugh at transphobia by demonstrating how ridiculous it is. The title character literally takes a toilet plunger to his face when he realizes the woman he kissed is a biological male. Jim Carrey even said, "That was an honest completely homophobic reaction from that character. It was basically making fun of homophobia. It’s ridiculous. I’ve got a plunger on my face because I kissed a dude. It’s ridiculous."